Featured Methods

Preparative Ultracentrifugation (PUC)

Preparative ultracentrifugation (PUC) is the gold standard method for lipoprotein fractionation. Medpace labs is the “go-to” lab for PUC analysis for drugs in development, with capability for this very specialized and technically difficult testing in all four Medpace global central lab locations for low-density lipoprotein and other lipoprotein classes. Medpace performs PUC in high volume with over 2,000 analyses monthly.

PUC testing isolates the following lipoproteins, allowing the quantification of cholesterol, triglycerides, apolipoproteins (AI, AII, AIV, B48, B100, CII, CIII, E), phospholipids, fatty acids, and other lipid specific analytes.

Lipoprotein Fractions Isolated

Chylomicron Fraction LDL(Low Density Lipoprotein) Fraction
VLDL (Very Low Density Lipoprotein) Fraction HDL (High Density Lipoprotein) Fraction
IDL (Intermediate Density Lipoprotein) Fraction HDL Sub-fractions HDL2 and HDL3

Chromatography

Medpace laboratories (central and bioanalytical labs) have broad experience in chromatographic assays supporting preclinical studies and Phase I-IV clinical trials.

Laboratory Services

Key Instrumentation

Bile Acids Nutritional Biomarkers, including AB Sciex 4000, 5500, 5600, and 6500 LC-MS/MS
Drug pharmacokinetics and metabolite profiling   Alpha Carotene Shimadzu 060 LC-MS/MS
Fatty Acid Panel   Beta Carotene Shimadzu GCMS-QP2010
Hemoglobin A1c (NGSP Level 1 accredited)   Vitamin A Tosoh G7
Retinyl Palmitate   Vitamin B Tosoh G8
Hormones, including:   Vitamin 25 OH D2and D3 Waters AcQuity UPLC
  Dihydrotestosterone (DHT)   Vitamin E Waters Xevo TQ-XS LC-MS/MS
  Estradiol/Estrone   Vitamin K1 and K2
  Testosterone Total / Free