Preparative Ultracentrifugation (PUC)
Preparative ultracentrifugation (PUC) is the gold standard method for lipoprotein fractionation. Medpace labs is the “go-to” lab for PUC analysis for drugs in development, with capability for this very specialized and technically difficult testing in all four Medpace global central lab locations for low-density lipoprotein and other lipoprotein classes. Medpace performs PUC in high volume with over 2,000 analyses monthly.
PUC testing isolates the following lipoproteins, allowing the quantification of cholesterol, triglycerides, apolipoproteins (AI, AII, AIV, B48, B100, CII, CIII, E), phospholipids, fatty acids, and other lipid specific analytes.
Lipoprotein Fractions Isolated
|Chylomicron Fraction||LDL(Low Density Lipoprotein) Fraction|
|VLDL (Very Low Density Lipoprotein) Fraction||HDL (High Density Lipoprotein) Fraction|
|IDL (Intermediate Density Lipoprotein) Fraction||HDL Sub-fractions HDL2 and HDL3|
PUC Scientific Posters Presented by Medpace
Friedewald Formula Significantly Underestimates LDL Cholesterol Compared to Preparative Ultracentrifugation below 70 mg/dL leading to Overestimation of the LDL Cholesterol Reduction for New drugs in Development
Accurate Assessment of LDL Cholesterol Reduction at Levels Below 70mg/dL Has Implications in the Estimation of Efficacy for New Drugs in Development
Underestimation of LDL Cholesterol Below 70 mg/dL (1.8 mmol/L) by Friedewald, Hopkins and a “Direct” Assay Compared to Preparative Ultracentrifugation Leading to the Overestimation of LDL Cholesterol Reduction for New Drugs in Development
LDL Cholesterol Reduction with BMS-962476, An Adnectin Inhibitor Of PCSK9: Results Of A Single Ascending Dose Study
Medpace laboratories (central and bioanalytical labs) have broad experience in chromatographic assays supporting preclinical studies and Phase I-IV clinical trials.
|Bile Acids||Nutritional Biomarkers, including||AB Sciex 4000, 5500, 5600, and 6500 LC-MS/MS|
|Drug pharmacokinetics and metabolite profiling||Alpha Carotene||Shimadzu 060 LC-MS/MS|
|Fatty Acid Panel||Beta Carotene||Shimadzu GCMS-QP2010|
|Hemoglobin A1c (NGSP Level 1 accredited)||Vitamin A||Tosoh G7|
|Retinyl Palmitate||Vitamin B||Tosoh G8|
|Hormones, including:||Vitamin 25 OH D2and D3||Waters AcQuity UPLC|
|Dihydrotestosterone (DHT)||Vitamin E||Waters Xevo TQ-XS LC-MS/MS|
|Estradiol/Estrone||Vitamin K1 and K2|
|Testosterone Total / Free|